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Cryo-Transmission Electron Microscopy of Frozen-Hydrated Sections of Escherichia coli and Pseudomonas aeruginosa

High-pressure freezing of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1 in the presence of cryoprotectants provided consistent vitrification of cells so that frozen-hydrated sections could be cut, providing ∼2-nm resolution of structure. The size and shape of the bacteria, as well as their s...

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Detalhes bibliográficos
Main Authors: Matias, Valério R. F., Al-Amoudi, Ashraf, Dubochet, Jacques, Beveridge, Terry J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC225031/
https://ncbi.nlm.nih.gov/pubmed/14526023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.20.6112-6118.2003
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