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His-tag protein monitoring by a fast mix-and-measure immunoassay
Here, we present a fast mix-and-measure immunoassay for the specific semiquantitative detection of His-tagged proteins, for example in E. coli cell lysate. The assay is based on Förster resonance energy transfer (FRET) between a lanthanide dye-labeled low-affinity His-peptide and an acceptor-labeled...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4085604/ https://ncbi.nlm.nih.gov/pubmed/25000910 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep05613 |
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