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Variability in the Immunodetection of His-tagged Recombinant Proteins
Labeling of recombinant proteins with polypeptide fusion partners, or affinity tagging, is a useful method to facilitate subsequent protein purification and detection. Poly-histidine tags (His-tags) are among the most commonly used affinity tags. We report strikingly variable immunodetection of two...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1821093/ https://ncbi.nlm.nih.gov/pubmed/17081490 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.09.017 |
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