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Determination of Rate Constants and Equilibrium Constants for Solution-Phase Drug–Protein Interactions by Ultrafast Affinity Extraction

[Image: see text] A method was created on the basis of ultrafast affinity extraction to determine both the dissociation rate constants and equilibrium constants for drug–protein interactions in solution. Human serum albumin (HSA), an important binding agent for many drugs in blood, was used as both...

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Detalhes bibliográficos
Main Authors: Zheng, Xiwei, Li, Zhao, Podariu, Maria I., Hage, David S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4082384/
https://ncbi.nlm.nih.gov/pubmed/24911267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac501031y
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