Generation of Transgenic Pigs by Cytoplasmic Injection of piggyBac Transposase-Based pmGENIE-3 Plasmids

The process of transgenesis involves the introduction of a foreign gene, the transgene, into the genome of an animal. Gene transfer by pronuclear microinjection (PNI) is the predominant method used to produce transgenic animals. However, this technique does not always result in germline transgenic o...

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Bibliographic Details
Main Authors: Li, Zicong, Zeng, Fang, Meng, Fanming, Xu, Zhiqian, Zhang, Xianwei, Huang, Xiaoling, Tang, Fei, Gao, Wenchao, Shi, Junsong, He, Xiaoyan, Liu, Dewu, Wang, Chong, Urschitz, Johann, Moisyadi, Stefan, Wu, Zhenfang
Format: Artigo
Language:Inglês
Published: Society for the Study of Reproduction, Inc. 2014
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4076374/
https://ncbi.nlm.nih.gov/pubmed/24671876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1095/biolreprod.113.116905
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