Efficient delivery of RNA interference oligonucleotides to polarized airway epithelia in vitro

Polarized and pseudostratified primary airway epithelia present barriers that significantly reduce their transfection efficiency and the efficacy of RNA interference oligonucleotides. This creates an impediment in studies of the airway epithelium, diminishing the utility of loss-of-function as a res...

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Autors principals: Ramachandran, Shyam, Krishnamurthy, Sateesh, Jacobi, Ashley M., Wohlford-Lenane, Christine, Behlke, Mark A., Davidson, Beverly L., McCray, Paul B.
Format: Artigo
Idioma:Inglês
Publicat: American Physiological Society 2013
Matèries:
Innovative Methodology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4073929/
https://ncbi.nlm.nih.gov/pubmed/23624792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajplung.00426.2012
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