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Technique for quantitative RT-PCR analysis directly from single muscle fibers

The use of single-cell quantitative RT-PCR has greatly aided the study of gene expression in fields such as muscle physiology. For this study, we hypothesized that single muscle fibers from a biopsy can be placed directly into the reverse transcription buffer and that gene expression data can be obt...

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Detalles Bibliográficos
Main Authors: Wacker, Michael J., Tehel, Michelle M., Gallagher, Philip M.
Formato: Artigo
Idioma:Inglês
Publicado: American Physiological Society 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4073910/
https://ncbi.nlm.nih.gov/pubmed/18467545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/japplphysiol.00897.2007
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