Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies

BACKGROUND: Due to the limited RNA amounts from endomyocardial biopsies (EMBs) and low expression levels of certain genes, gene expression analyses by conventional real-time RT-PCR are restrained in EMBs. We applied two preamplification techniques, the TaqMan(® )PreAmp Master Mix (T-PreAmp) and a mu...

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Библиографические подробности
Главные авторы: Noutsias, Michel, Rohde, Maria, Block, Andrea, Klippert, Katrin, Lettau, Olga, Blunert, Katja, Hummel, Michael, Kühl, Uwe, Lehmkuhl, Hans, Hetzer, Roland, Rauch, Ursula, Poller, Wolfgang, Pauschinger, Matthias, Schultheiss, Heinz P, Volk, Hans D, Kotsch, Katja
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2008
Предметы:
Methodology Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2262094/
https://ncbi.nlm.nih.gov/pubmed/18194512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-9-3
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