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Technique for quantitative RT-PCR analysis directly from single muscle fibers
The use of single-cell quantitative RT-PCR has greatly aided the study of gene expression in fields such as muscle physiology. For this study, we hypothesized that single muscle fibers from a biopsy can be placed directly into the reverse transcription buffer and that gene expression data can be obt...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Physiological Society
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4073910/ https://ncbi.nlm.nih.gov/pubmed/18467545 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/japplphysiol.00897.2007 |
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