CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

CRISPR/Cas9 systems are a versatile tool for genome editing due to the highly efficient targeting of DNA sequences complementary to their RNA guide strands. However, it has been shown that RNA-guided Cas9 nuclease cleaves genomic DNA sequences containing mismatches to the guide strand. A better unde...

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Hlavní autoři: Lin, Yanni, Cradick, Thomas J., Brown, Matthew T., Deshmukh, Harshavardhan, Ranjan, Piyush, Sarode, Neha, Wile, Brian M., Vertino, Paula M., Stewart, Frank J., Bao, Gang
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2014
Témata:
Synthetic Biology and Chemistry
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066799/
https://ncbi.nlm.nih.gov/pubmed/24838573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku402
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