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Rapid isolation of yeast genomic DNA: Bust n' Grab

BACKGROUND: Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either glass beads or enzymatic digestion to disrupt yeast cell wall. Using small glass...

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Detalhes bibliográficos
Main Authors: Harju, Susanna, Fedosyuk, Halyna, Peterson, Kenneth R
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC406510/
https://ncbi.nlm.nih.gov/pubmed/15102338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-4-8
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