Isolation of single-base genome-edited human iPS cells without antibiotic selection

Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-...

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Hlavní autoři: Miyaoka, Yuichiro, Chan, Amanda H., Judge, Luke M., Yoo, Jennie, Huang, Miller, Nguyen, Trieu D., Lizarraga, Paweena P., So, Po-Lin, Conklin, Bruce R.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4063274/
https://ncbi.nlm.nih.gov/pubmed/24509632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2840
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