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The extracellular protein VlsE is destabilized inside cells
We use U2OS cells as in vivo ‘test tubes’ to study how the same cytoplasmic environment has opposite effects on the stability of two different proteins. Protein folding stability and kinetics were compared by Fast Relaxation Imaging (FReI), which combines a temperature jump with fluorescence microsc...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4059009/ https://ncbi.nlm.nih.gov/pubmed/24013077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2013.08.024 |
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