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TECHNICAL BRIEF: Optimized pipeline for isolation of high-quality RNA from corneal cell subpopulations

Purpose: Attempts to determine the transcriptional profile of discrete subsets of limbal epithelial cells in situ using laser capture microdissection (LCM) face two major challenges. First, the transcriptional profile of cells within a tissue may rapidly change as the tissue is excised and exposed t...

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Bibliografiske detaljer
Main Authors: Bath, Chris, Fink, Trine, Vorum, Henrik, Hjortdal, Jesper, Zachar, Vladimir
Format: Artigo
Sprog:Inglês
Udgivet: Molecular Vision 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4057246/
https://ncbi.nlm.nih.gov/pubmed/24940035
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