Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

BACKGROUND: Transcriptomic profiling of ex vivo cultured human limbal epithelial stem cells (hLESCs) will foster better understanding of corneal physiology and novel treatment paradigms to limbal stem cell deficiency (LSCD). However, currently such profiling studies are hampered due to difficulties...

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Detalles Bibliográficos
Publicado en:Biol Proced Online
Main Authors: Liu, Lei, Nielsen, Frederik Mølgaard, Riis, Simone Elkjær, Emmersen, Jeppe, Fink, Trine, Hjortdal, Jesper Østergaard, Bath, Chris, Zachar, Vladimir
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2017
Assuntos:
Methodology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727887/
https://ncbi.nlm.nih.gov/pubmed/29255379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-017-0065-2
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