Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

BACKGROUND: Transcriptomic profiling of ex vivo cultured human limbal epithelial stem cells (hLESCs) will foster better understanding of corneal physiology and novel treatment paradigms to limbal stem cell deficiency (LSCD). However, currently such profiling studies are hampered due to difficulties...

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Bibliografiska uppgifter
I publikationen:Biol Proced Online
Huvudupphovsmän: Liu, Lei, Nielsen, Frederik Mølgaard, Riis, Simone Elkjær, Emmersen, Jeppe, Fink, Trine, Hjortdal, Jesper Østergaard, Bath, Chris, Zachar, Vladimir
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2017
Ämnen:
Methodology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727887/
https://ncbi.nlm.nih.gov/pubmed/29255379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-017-0065-2
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