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TECHNICAL BRIEF: Optimized pipeline for isolation of high-quality RNA from corneal cell subpopulations

Purpose: Attempts to determine the transcriptional profile of discrete subsets of limbal epithelial cells in situ using laser capture microdissection (LCM) face two major challenges. First, the transcriptional profile of cells within a tissue may rapidly change as the tissue is excised and exposed t...

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Detalhes bibliográficos
Main Authors: Bath, Chris, Fink, Trine, Vorum, Henrik, Hjortdal, Jesper, Zachar, Vladimir
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Vision 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4057246/
https://ncbi.nlm.nih.gov/pubmed/24940035
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