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Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones
CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitt...
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| Main Authors: | , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
International Union of Crystallography
2014
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4051524/ https://ncbi.nlm.nih.gov/pubmed/24915080 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14008516 |
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