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Structure of a His170Tyr mutant of thermostable pNPPase from Geobacillus stearothermophilus

Using directed evolution based on random mutagenesis and heat-treated selection, a thermostable His170Tyr mutant of Geobacillus stearothermophilus thermostable p-nitrophenylphosphatase (TpNPPase) was obtained. The temperature at which the His170Tyr mutant lost 50% of its activity (T (1/2)) was found...

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Bibliografiske detaljer
Main Authors: Shen, Tiantian, Guo, Zheng, Ji, Chaoneng
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4051519/
https://ncbi.nlm.nih.gov/pubmed/24915075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14007341
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