Cell cycle synchronization of leukemia inhibitory factor (LIF)-dependent porcine-induced pluripotent stem cells and the generation of cloned embryos

Nuclear transfer (NT) from porcine iPSC to create cloned piglets is unusually inefficient. Here we examined whether such failure might be related to the cell cycle stage of donor nuclei. Porcine iPSC, derived here from the inner cell mass of blastocysts, have a prolonged S phase and are highly sensi...

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Detalhes bibliográficos
Main Authors: Yuan, Ye, Lee, Kiho, Park, Kwang-Wook, Spate, Lee D, Prather, Randall S, Wells, Kevin D, Roberts, R Michael
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4049963/
https://ncbi.nlm.nih.gov/pubmed/24621508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/cc.28176
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