Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system

Labeling and tracing of specific sequences in living cells has been a major challenge in studying the spatiotemporal dynamics of native chromatin. Here we repurposed the prokaryotic CRISPR/Cas adaptive immunity system to specifically detect endogenous genomic loci in mouse embryonic stem cells. We c...

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Bibliographic Details
Main Authors: Anton, Tobias, Bultmann, Sebastian, Leonhardt, Heinrich, Markaki, Yolanda
Format: Artigo
Language:Inglês
Published: Landes Bioscience 2014
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Research Paper
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4049922/
https://ncbi.nlm.nih.gov/pubmed/24637835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/nucl.28488
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