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Comparing mRNA levels using in situ hybridization of a target gene and co-stain

In situ hybridization is an important technique for measuring the spatial expression patterns of mRNA in cells, tissues, and whole animals. However, mRNA levels cannot be compared across experiments using typical protocols. Here we present a semi-quantitative method to compare mRNA levels of a gene...

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Hlavní autoři: Wunderlich, Zeba, Bragdon, Meghan D, DePace, Angela H
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4048779/
https://ncbi.nlm.nih.gov/pubmed/24434507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2014.01.003
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