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Comparing mRNA levels using in situ hybridization of a target gene and co-stain
In situ hybridization is an important technique for measuring the spatial expression patterns of mRNA in cells, tissues, and whole animals. However, mRNA levels cannot be compared across experiments using typical protocols. Here we present a semi-quantitative method to compare mRNA levels of a gene...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4048779/ https://ncbi.nlm.nih.gov/pubmed/24434507 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2014.01.003 |
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