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Studies of Single-Chain Antibody Expression in Quiescent Escherichia coli
Quiescent Escherichia coli cells are generated by overexpressing the Rcd transcript in an hns-205 mutant host. The resulting nongrowing, metabolically active cells were used here to express a single-chain antibody fragment (scFv) in shake flask and fermentor cultures. The expression system is based...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC404426/ https://ncbi.nlm.nih.gov/pubmed/15128562 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.5.3005-3012.2004 |
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