Studies of Single-Chain Antibody Expression in Quiescent Escherichia coli

Quiescent Escherichia coli cells are generated by overexpressing the Rcd transcript in an hns-205 mutant host. The resulting nongrowing, metabolically active cells were used here to express a single-chain antibody fragment (scFv) in shake flask and fermentor cultures. The expression system is based...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Mukherjee, K. J., Rowe, D. C. D., Watkins, N. A., Summers, D. K.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2004
Subjects:
Physiology and Biotechnology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404426/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15128562/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.5.3005-3012.2004
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