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Site-Specific Bioconjugation of a Murine Dihydrofolate Reductase Enzyme by Copper(I)-Catalyzed Azide-Alkyne Cycloaddition with Retained Activity

Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is an efficient reaction linking an azido and an alkynyl group in the presence of copper catalyst. Incorporation of a non-natural amino acid (NAA) containing either an azido or an alkynyl group into a protein allows site-specific bioconjugation in m...

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Detalhes bibliográficos
Main Authors: Lim, Sung In, Mizuta, Yukina, Takasu, Akinori, Kim, Yong Hwan, Kwon, Inchan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4041766/
https://ncbi.nlm.nih.gov/pubmed/24887377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0098403
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