Multichange Isothermal Mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA

[Image: see text] Multichange ISOthermal (MISO) mutagenesis is a new technique allowing simultaneous introduction of multiple site-directed mutations into plasmid DNA by leveraging two existing ideas: QuikChange-style primers and one-step isothermal (ISO) assembly. Inversely partnering pairs of Quik...

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Main Authors: Mitchell, Leslie A., Cai, Yizhi, Taylor, Martin, Noronha, Anne Marie, Chuang, James, Dai, Lixin, Boeke, Jef D.
Format: Artigo
Jezik:Inglês
Izdano: 2013
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4040258/
https://ncbi.nlm.nih.gov/pubmed/23654272
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb300131w
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