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Multichange Isothermal Mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA
[Image: see text] Multichange ISOthermal (MISO) mutagenesis is a new technique allowing simultaneous introduction of multiple site-directed mutations into plasmid DNA by leveraging two existing ideas: QuikChange-style primers and one-step isothermal (ISO) assembly. Inversely partnering pairs of Quik...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4040258/ https://ncbi.nlm.nih.gov/pubmed/23654272 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb300131w |
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