Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

The CRISPR/Cas9 system has been implemented in a variety of model organisms to mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at a limited number of genomic target sites. To uncover the rules that govern effective Cas9-mediated mutagenesis in zebrafish, we t...

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Detalhes bibliográficos
Main Authors: Gagnon, James A., Valen, Eivind, Thyme, Summer B., Huang, Peng, Ahkmetova, Laila, Pauli, Andrea, Montague, Tessa G., Zimmerman, Steven, Richter, Constance, Schier, Alexander F.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4038517/
https://ncbi.nlm.nih.gov/pubmed/24873830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0098186
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