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Redesigning enzymes based on adaptive evolution for optimal function in synthetic metabolic pathways

Nature has balanced most metabolic pathways such that no one enzyme in the pathway controls the flux through that pathway. However, unnatural or non-native, constructed metabolic pathways may have limited product flux due to unfavorable in vivo properties of one or more enzymes in the pathway. One s...

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Autors principals: Yoshikuni, Yasuo, Dietrich, Jeffrey A., Nowroozi, Farnaz F., Babbitt, Patricia C., Keasling, Jay D.
Format: Artigo
Idioma:Inglês
Publicat: 2008
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4030648/
https://ncbi.nlm.nih.gov/pubmed/18559271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2008.05.006
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