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Redesigning enzymes based on adaptive evolution for optimal function in synthetic metabolic pathways

Nature has balanced most metabolic pathways such that no one enzyme in the pathway controls the flux through that pathway. However, unnatural or non-native, constructed metabolic pathways may have limited product flux due to unfavorable in vivo properties of one or more enzymes in the pathway. One s...

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Detalles Bibliográficos
Main Authors: Yoshikuni, Yasuo, Dietrich, Jeffrey A., Nowroozi, Farnaz F., Babbitt, Patricia C., Keasling, Jay D.
Formato: Artigo
Idioma:Inglês
Publicado: 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4030648/
https://ncbi.nlm.nih.gov/pubmed/18559271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2008.05.006
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