Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination.
DNA molecules undergoing transformation into yeast are highly recombinogenic, even when diverged. We reasoned that transformation-associated recombination (TAR) could be employed to clone large DNAs containing repeat sequences, thereby eliminating the need for in vitro enzymatic reactions such as re...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40264/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8552668/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.1.491 |
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