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High-Throughput Screening of Effective siRNAs Using Luciferase-Linked Chimeric mRNA
The use of siRNAs to knock down gene expression can potentially be an approach to treat various diseases. To avoid siRNA toxicity the less transcriptionally active H1 pol III promoter, rather than the U6 promoter, was proposed for siRNA expression. To identify highly efficacious siRNA sequences, ext...
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| Auteurs principaux: | , , , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science
2014
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4022502/ https://ncbi.nlm.nih.gov/pubmed/24831610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096445 |
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