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Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate and EDTA (TAE) or Tris, borate and EDTA (TBE). Gels are run at a low, constant voltage (~ 10 V/cm) to minimize current and asymmetric heating effects, which can induce band artifacts and p...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4021863/ https://ncbi.nlm.nih.gov/pubmed/24637158 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.03.003 |
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