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Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits(2). During gelation, agarose poly...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Lee, Pei Yun, Costumbrado, John, Hsu, Chih-Yuan, Kim, Yong Hoon
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4846332/
https://ncbi.nlm.nih.gov/pubmed/22546956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3923
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