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Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits(2). During gelation, agarose poly...

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Publicado en:J Vis Exp
Autores principales: Lee, Pei Yun, Costumbrado, John, Hsu, Chih-Yuan, Kim, Yong Hoon
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4846332/
https://ncbi.nlm.nih.gov/pubmed/22546956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3923
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