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Electrophoresis and orientation of F-actin in agarose gels.

F-Actin was electrophoresed on agarose gels. In the presence of 2 mM MgCl2 and above pH 8.5 F-actin entered 1% agarose; when the electric field was 2.1 V/cm and the pH was 8.8, F-actin migrated through a gel as a single band at a rate of 2.5 mm/h. Labeling of actin with fluorophores did not affect i...

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Bibliografski detalji
Glavni autori: Borejdo, J, Ortega, H
Format: Artigo
Jezik:Inglês
Izdano: 1989
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1280478/
https://ncbi.nlm.nih.gov/pubmed/2528384
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