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A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity
BACKGROUND: Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA...
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| Principais autores: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4021065/ https://ncbi.nlm.nih.gov/pubmed/24694320 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-15-7 |
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