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A new reverse transcription-polymerase chain reaction method for accurate quantification
BACKGROUND: Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. Specific genes ha...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2003
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC317330/ https://ncbi.nlm.nih.gov/pubmed/14664723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-3-22 |
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