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A new reverse transcription-polymerase chain reaction method for accurate quantification
BACKGROUND: Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. Specific genes ha...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2003
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC317330/ https://ncbi.nlm.nih.gov/pubmed/14664723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-3-22 |
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