Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization

BACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for...

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Detalhes bibliográficos
Main Authors: Ho-Pun-Cheung, Alexandre, Bascoul-Mollevi, Caroline, Assenat, Eric, Boissière-Michot, Florence, Bibeau, Frédéric, Cellier, Dominic, Ychou, Marc, Lopez-Crapez, Evelyne
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2679744/
https://ncbi.nlm.nih.gov/pubmed/19368728
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-10-31
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