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Measurement of Protein Kinase B Activity in Single Primary Human Pancreatic Cancer Cells

[Image: see text] An optimized peptide substrate was used to measure protein kinase B (PKB) activity in single cells. The peptide substrate was introduced into single cells, and capillary electrophoresis was used to separate and quantify nonphosphorylated and phosphorylated peptide. The system was v...

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Autori principali: Proctor, Angela, Herrera-Loeza, S. Gabriela, Wang, Qunzhao, Lawrence, David S., Yeh, Jen Jen, Allbritton, Nancy L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2014
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4018172/
https://ncbi.nlm.nih.gov/pubmed/24716819
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac500616q
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