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A Capillary Electrophoresis Sequencing Method for the Identification of Mutations in the Inverted Terminal Repeats of Adeno-Associated Virus

Inverted terminal repeat (ITR) integrity is critical for the replication, packaging, and transduction of recombinant adeno-associated virus (rAAV), a promising gene therapy vector. Because AAV ITRs possess 70% GC content and are palindromic, they are notoriously difficult to sequence. The purpose of...

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Autores principales: Mroske, Cameron, Rivera, Hector, Ul-Hasan, Taihra, Chatterjee, Saswati, Wong, K.K.
Formato: Artigo
Lenguaje:Inglês
Publicado: Mary Ann Liebert, Inc. 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4015221/
https://ncbi.nlm.nih.gov/pubmed/22612656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2011.231
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