Cloning, Expression and Characterization of a Novel Thermophilic Polygalacturonase from Caldicellulosiruptor bescii DSM 6725

We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optima...

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Main Authors: Chen, Yanyan, Sun, Dejun, Zhou, Yulai, Liu, Liping, Han, Weiwei, Zheng, Baisong, Wang, Zhi, Zhang, Zuoming
Formato: Artigo
Idioma:Inglês
Publicado: Molecular Diversity Preservation International (MDPI) 2014
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013591/
https://ncbi.nlm.nih.gov/pubmed/24705464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms15045717
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