Cloning, Expression and Characterization of a Novel Thermophilic Polygalacturonase from Caldicellulosiruptor bescii DSM 6725

We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optima...

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Detalhes bibliográficos
Main Authors: Chen, Yanyan, Sun, Dejun, Zhou, Yulai, Liu, Liping, Han, Weiwei, Zheng, Baisong, Wang, Zhi, Zhang, Zuoming
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International (MDPI) 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013591/
https://ncbi.nlm.nih.gov/pubmed/24705464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms15045717
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