Cloning, Expression and Characterization of a Novel Thermophilic Polygalacturonase from Caldicellulosiruptor bescii DSM 6725

We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optima...

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Hauptverfasser: Chen, Yanyan, Sun, Dejun, Zhou, Yulai, Liu, Liping, Han, Weiwei, Zheng, Baisong, Wang, Zhi, Zhang, Zuoming
Format: Artigo
Sprache:Inglês
Veröffentlicht: Molecular Diversity Preservation International (MDPI) 2014
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013591/
https://ncbi.nlm.nih.gov/pubmed/24705464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms15045717
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