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Flavodoxin Cofactor Binding Induces Structural Changes that are Required for Protein-Protein Interactions with NADP(+) Oxidoreductase and Pyruvate Formate-Lyase Activating Enzyme

Flavodoxin (Fld) conformational changes, thermal stability, and cofactor binding were studied using circular dichroism (CD), isothermal titration calorimetry (ITC), and limited proteolysis. Thermodynamics of apo and holo-Fld folding were examined to discern the features of this important electron tr...

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Detalhes bibliográficos
Main Authors: Crain, Adam V., Broderick, Joan B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4012331/
https://ncbi.nlm.nih.gov/pubmed/24016774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2013.08.014
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