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A combinatorial mutagenesis approach for functional epitope mapping on phage-displayed target antigen: Application to antibodies against epidermal growth factor

Although multiple different procedures to characterize the epitopes recognized by antibodies have been developed, site-directed mutagenesis remains the method of choice to define the energetic contribution of antigen residues to binding. These studies are useful to identify critical residues and to...

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Autori principali: Infante, Yanelys Cabrera, Pupo, Amaury, Rojas, Gertrudis
Natura: Artigo
Lingua:Inglês
Pubblicazione: Landes Bioscience 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4011908/
https://ncbi.nlm.nih.gov/pubmed/24589624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.28395
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