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Helicase and polymerase move together close to the fork junction and copy DNA in one-nucleotide steps
By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading strand synthesis taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound-copied. The cooperative catalysis enables the helicase and polymer...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4010093/ https://ncbi.nlm.nih.gov/pubmed/24630996 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2014.02.025 |
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