Identification of residues essential for catalysis and binding of calmodulin in Bordetella pertussis adenylate cyclase by site-directed mutagenesis.

In order to identify molecular features of the calmodulin (CaM) activated adenylate cyclase of Bordetella pertussis, a truncated cya gene was fused after the 459th codon in frame with the alpha-lacZ' gene fragment and expressed in Escherichia coli. The recombinant, 604 residue long protein was...

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Hauptverfasser: Glaser, P, Elmaoglou-Lazaridou, A, Krin, E, Ladant, D, Bârzu, O, Danchin, A
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC400898/
https://ncbi.nlm.nih.gov/pubmed/2542030
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