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Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.

An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the full-length Ad genome cloned in a cassette cosmid. The cassette bearing the expression unit was then...

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Publicado no:Proc Natl Acad Sci U S A
Principais autores: Miyake, S, Makimura, M, Kanegae, Y, Harada, S, Sato, Y, Takamori, K, Tokuda, C, Saito, I
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1996
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40078/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8577762/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.3.1320
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