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Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

BACKGROUND: Segregational stability of plasmids is of major concern for recombinant bacterial production strains. One of the best strategies to counteract plasmid loss is the use of auxotrophic mutants which are complemented with the lacking gene along with the product-relevant ones. However, these...

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Detalhes bibliográficos
Main Authors: Velur Selvamani, Ram Shankar, Telaar, Maurice, Friehs, Karl, Flaschel, Erwin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4006690/
https://ncbi.nlm.nih.gov/pubmed/24745552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-13-58
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